中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻中一个与OsHSP90互作的新基因特性分析

马炳田1, 2,#,万佳3,#,张 琦2,#,江 斌1,徐永菊1,李冬梅1,徐正君1,2,*   

  1. 1四川农业大学 水稻研究所, 四川 温江 611130; 2四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014; 3四川省农业厅植物检疫站, 四川 成都 610041; ﹟共同第一作者;*通讯联系人, E-mail: mywildrice@yahoo.com.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-10 发布日期:2009-07-10

Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

MA Bingtian1, 2,﹟, WAN Jia3,﹟, ZHANG Qi2,﹟, JIANG Bin1, XU Yongju1, LI Dongmei1, XU Zhengjun1, 2, *   

  1. 1 Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; 2 Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China; 3 Plant Quarantine Station of Sichuan Agriculture Department, Chengdu 610041, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: mywildrice@yahoo.com.cn
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-10 Published:2009-07-10

摘要: OsHSP90是水稻中一类可以在不同逆境条件下被诱导的基因。利用酵母双杂交系统,在水稻低温和干旱cDNA文库中筛选出1个与OsHSP90互作的未知基因,命名为OsHSPBP。序列分析表明,该基因全长1321 bp,编码一个具有244个氨基酸残基的多肽,推测分子量为25.5 kD。在OsHSPBP蛋白的97-132位氨基酸残基间存在tify功能域,185-211位氨基酸残基构成CCT_2基序,与其他植物中的同源蛋白相似性介于30.66%~70.40%。经分析,该基因启动子区域存在6种不同的逆境反应顺式作用元件。RTPCR半定量结果表明, OsHSP90与OsHSPBP在逆境胁迫下,均能被不同程度地诱导并呈现相似的表达趋势,说明两者可能与水稻逆境胁迫的应答和信号传递有关。

关键词: 水稻, 逆境胁迫, 诱导基因, 基因表达, 氨基酸序列, 酵母双杂交系统

Abstract: OsHSP90 is an abiotic stressinducible gene in rice. From the chilling and droughtinducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSP90 was identified by using yeast twohybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame (ORF) predicts a 244amino acid(aa) polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185-211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66%-70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to ciselements related to the abiotic stress response were identified using the PlantCARE software. Semiquantitative RTPCR showed that the OsHSP90 and OsHSPBP might be detected in the similar degree under different stresstreatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.

Key words: rice, abiotic stress, inducible gene, gene expression, amino acid sequence, yeast twohybrid system